dsRNA Analyzer is a computational pipeline that facilitates the design of dsRNAs with optimized specificity in a target organism (TO, i.e. a pest) and minimized off-target potential in non-target organisms (NTOs, e.g. bees, humans). dsRNA Analyzer systematically evaluates all predicted small interfering RNAs (siRNAs) derived from an input nucleotide target sequence for their potential to elicit gene-specific knockdown. Using this information, it then proposes candidate dsRNA molecules. Customizable parameters include the desired length of the predicted siRNAs and that of the suggested dsRNA molecule and the number of allowed mismatches when screening on NTOs.

The output comprises tabulated reports that help the researcher or regulator design a dsRNA molecule with great specificity on the TO and minimal or no hits on any NTO. dsRNA Analyzer also outputs the specific patterns of any undesired siRNA hits on the NTOs providing a basis for selecting dsRNA sequences with optimal trade-offs between on-target potency and ecotoxicological safety. dsRNA Analyzer is unique in its ability to test both the genome or transcriptome of an NTO for unwanted hits.

The current implementation includes an integrated reference database of more than 90 NTO genomes and over 70 NTO transcriptomes, covering a broad range of taxonomic groups relevant to regulatory biosafety evaluations.

dsRNA Analyzer can be run as a web app at  http://dsrna-analyzer.org:3000/dsrna-analyzer

In the frame of the project, we have developed a new open access pipeline which integrates a series of up-to-date software offering:

• High Accuracy in strain identification
• Detection of pathogenic potency through the identification of virulence factors
• Detection of antibiotic resistance genes, localization in the genome and potency for transferability

Detection and description of gene clusters associated with the biosynthesis of secondary metabolites of concern

The pipeline can be used for the analysis of both bacterial and fungal genomes and provides all necessary information for risk assessment of microbials. You can access the tool and test it in the following link upon registration:
https://agrostis.gr/ration-ra-gui/#/login

RATION has developed its own app which you could download easily and have access to all updated information on biopesticides, which ones are registered on which countries and which ones are pending registration. You could register as user of biopesticides their use helping us to gather data on their use per country.

Download it in: https://play.google.com/store/apps/details?id=gr.agrostis.biopesticides